Journal of Nutrition and Health Sciences

ISSN: 2393-9060

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A Review on Antioxidant, Anti-Inflammatory and Gastroprotective Abilities of Mango (Magnifera indica) Leaf Extract and Mangiferin

Received Date: March 18, 2018 Accepted Date: September 11, 2018 Published Date: September 13, 2018

Copyright: © 2018 Stohs SJ. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Mango (Mangifera indica Linn.) trees have been cultivated and grown in Southern and Eastern India for thousands of years and have slowly spread all over the world. For over 4,000 years various parts of mango trees including leaf and fruit pulp have been extensively used in Ayurvedic and Unani medicines. Mango pulp and leaves contain diverse health promoting chemical constituents including mangiferin, a natural polyphenol of C-glycosylxanthone structure, with diverse pharmacological/physiological activities including the promotion of gut health and regularity, as well as antioxidant and anti-inflammatory benefits. This review provides a detailed discussion of the nutritional benefits of mango leaf extract and mangiferin in promoting anti-inflammatory, antioxidant, gastrointestinal and gut health benefits. In addition, information regarding the tissue protective, immunomodulatory, antidiabetic, anti-hyperlipidemic and anti-cancer properties, as well as for disease management and health benefits of mango leaf extracts and mangiferin will be reviewed. Furthermore, mechanistic studies involving the free radical scavenging ability of mangiferin as well as its ability to exert anti-inflammatory activity by inhibiting the expression of the TNF-α and related signaling mechanisms and inducible nitric oxide synthase will be considered.

Keywords: Mango Fruit and Leaves; Mangiferin; Anti-inflammatory; Antioxidant; Gastro-Intestinal and Gut Health; RegularityMango

List of abbreviations: ALT: Alanine Aminotransferase; AMP: Adenosine Monophosphate; AMPK: AMP Activated Protein Kinase; ASC: Apoptosis-associated Speck-Like Protein Containing a Caspase-Recruitment Domain; AST: Aspartate Aminotransferase; ATP: Adenosine Triphosphate; Bcl-2: B-cell Lymphoma 2; BUN: Blood Urea Nitrogen; CD73: Cluster of Differentiation 73; DOX: Doxorubicin; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ERK5: Extracellular-signal-regulated kinase 5; GTP: Guanosine-5’-triphosphate; HO-1: Hemeoxygenase-1; IBD: Inflammatory Bowel Disease; ICAM-1: Intercellular Cell Adhesion Molecule-1; IКBα: Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B-cells Inhibitor, Alpha; IL-1β: Interleukin-1beta; IL-6: Interleukin 6; LPS: Lipopolysaccharide; MAPK: Mitogen-activated Protein Kinase; MCP-1: Monocyte Chemoattractant Protein-1; MDA: Malondialdehyde; MMP-9: Matrix Metallopeptidase 9; MPO: Myeloperoxidase; NF-Кβ: Nuclear Factor Kappa-light-chain-enhancer of Activated B Cells; NLRP3: Nucleotide-binding domain, Leucine-rich-containing Family, Pyrin Domain-containing-3; NMR: Nuclear Magnetic Resonance; NO: Nitric Oxide; RANTES: Regulated on Activation, Normal T Cell Expressed and Secreted; TBARS: Thiobarbituric Acid Reactive Substances; TNF-α: Tumor Necrosis Factor Alpha; TNBS: 2,4,6-Trinitrobenzensulfonic Acid; TPGS: D-α-Tocopherol Polyethylene Glycol 1000 Succinate


According to Ayurveda and indigenous medical systems, mango [Mangifera indica Linn. family Anacardiaceae] is a tropical fruit with a distinctive nutritional and phytochemical composition [1,2]. Earlier literature recommends mango leaves, bark, seeds, flowers, and raw and ripe fruits for a myriad of medicinal, pharmacological and health benefits [1-5]. In addition, mango leaf is considered holy and sacred, and used as an integral part of all Hindu rituals from the Vedic ages. Research studies indicate that mango leaves and fruits exhibit antioxidant, anti-inflammatory, anti-diabetic, cardioprotective, anti-tumor, wound healing, anti-pyretic, anti-bacterial, anti-spasmodic, anti-carcinogenic, anti-viral, anti-microbial and anti-dysentry benefits, as well as hepatoprotective, gastroprotective, immunomodulatory and hypolipidemic effects [1-5]. The juice of ripe mango is purported to be beneficial against heat stroke, a fatal life-threatening inflammatory response [1,2,4,5].

Mango trees have been cultivated in the Indian sub-continent for thousands of years. They have been grown in Southeast Asia from the fifth and fourth centuries BC, and have been cultivated in East and West Africa since around the 10th century AD. Subsequently, cultivation of mangoes has occurred in Brazil, Mexico, West Indies and Caribbean Islands [6,7]. Mangos are also grown in Southern Europe, especially in the coastal subtropical environment of Spain, Canary Islands and Portugal. Alphonso mango is the most popular mango species grown in India, which is now exported worldwide as a fruit or juice. In 2012, the United Nations statistics indicated that India is the largest producer of mango (16.2 million tons), while China and Thailand produced approximately 4.4 and 3.0 million tons, respectively [1,6,7].

Mangoes are also used for various culinary purposes. Raw mangoes are sour and used in pickles and salad, while ripe mangoes are generally sweet and used in yogurt, juices, smoothies, pies, and milk shakes [1,2,8]. The purpose of this review is to provide an assessment primarily of the gastrointestinal effects of mango leaf extracts and mangiferin, the primary active constituent thereof, as well as their mechanisms of action.

Mango and Mango Leaf Constituents1

Mango leaves has been demonstrated to contain mangiferin (Figure 1), selected anthocyanidins including delphinidin, peonidin and cyanidin, leucoanthocyanins, catechin and gallic tannins, the mangiferin content of mango leaves ranges from about 2-15% depending on the variety and geographic source, while extracts of mango leaves contain up to approximately 60% mangiferin. Mango leaf oil is rich in sesquiterpenes and also contains mangiferin, δ-3-carene, α-gurjunene, β-selinene and β-caryophyllene. A phytochemical analysis reported that mango leaves contain alkaloids (0.84±0.11 mg/100 g), phenols (0.09±0.20 mg/100 g), flavonoids (11.24±0.10 mg/100 g), saponins (3.22±0.10 mg/100 g) and tannins (0.45±0.10 mg/100 g) [9,10]. Thus, mango leaves contain high amounts of polyphenolic antioxidants including xanthonoids, mangiferin and gallic acid [9,10].

Yusukol et al. developed an enzyme-linked immunosorbent assay [ELISA] for mangiferin and examined the mangiferin content of the mature leaves of 50 mango cultivars. The mangiferin content ranged from 1.94 to 13.79 mg/g (%) [11]. An Indian Thawai cultivar exhibited the highest content while a Namdokmai cultivar from Thailand contained 12.41 mg/g. Sharma et al. assessed the mangiferin content of aqueous extracts of 30 varieties of mango from west India using HPLC and UV detection [12]. The average mangiferin content from these varieties was 17.5 mg/g with the highest content from a Ladvo variety being 47.02 mg/g.

Mango leaves have also been shown to contain calcium (3.82±0.10 mg/100 g), magnesium (0.91±0.20 mg/100 g), potassium (0.83±0.10 mg/100 g), sodium (0.38±0.11 mg/100 g), zinc (7.88±0.10 mg/100 g), cadmium (1.50±0.20 mg/100 g), copper (8.68±0.10 mg/100 g) and phosphorus (0.78±0.10 mg/100 g) [1]. Furthermore, mango leaves are a good source of ascorbic acid (29.92±0.11 mg/100 g), riboflavin (0.09±0.10 mg/100 g), niacin (0.75±0.20 mg/100 g) and thiamine (0.45±0.11 mg/100 g) [9].

Chemistry and Structure of Mangiferin

A growing body of evidence suggests the significant potential of mangiferin (2C-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyxanthone; molecular formula C19H18O11; CAS 4773-96-0; molecular weight 422.34), a xanthone-C-glycoside present in diverse plant species, for versatile anti-inflammatory responses, potent antioxidant, radioprotective, immunomodulatory and anti-cancer properties, as well as for disease management and health benefits [8-21]. The antioxidant efficacy of mangiferin is stronger than cimmamon [1,13-16]. Furthermore, mangiferin exerts a pro-hypoglycemic activity by modulating glucose metabolism, ameliorating insulin resistance, lowering cholesterol synthesis, and inhibiting the expression of the TNF-α and inducible nitric oxide synthase [13-21].

Chemically, mangiferin is composed of a glucose unit C1→2 chemically bonded to a 1,3,6,7-tetrahydroxyxanthone aglycone, and lately it has been recognized as a potential pharmacophore. It is a light yellow color crystalline powder, slightly soluble in ethyl alcohol, sparingly soluble in methyl alcohol and water, and insoluble in diethyl ether, acetone and hexane [1,13,14]. It has also been reported that the solubility of mangiferin decreases with increasing temperature in the order ethanol > methanol > water > diethyl ether > acetone > n-hexane [1,10]. Somewhat in contrast to these observations, the ability of high pressure techniques to extract mangiferin lead to the following results [22]. Extraction of leaves with supercritical CO2 results in mangiferin extraction of about 1 mg/g, while adding ethanol to supercritical CO2 increases the yield to an average of 6.5 mg/g. However, when using a pressurized water extraction at 100 °C for 3 hours, the average yield was approximately 29 mg/g, providing an economically feasible system for extraction of mangiferin on an industrial scale.

Safety and Toxicity Studies of Mango Leaf Extract

A 14 day acute oral toxicity study of an aqueous ethanol extract of dried mango leaves standardized to 62% mangiferin was conducted in 40 male and female ICR mice (body weight 18-21 grams) [23]. No signs of toxicity or toxic manifestations were observed at maximum oral dose of 18.4 g mango leaf extract/kg bodyweight (11.4 g mangiferin/kg) twice a day with a 4 h interval between dosing. The animals were observed for 14 days for toxic reactions and mortality. At the end of the 14 days, all animals were healthy with no abnormal responses or lesions [23].

Another study demonstrated no signs of toxicity at a single oral dose of 5,000 mg of a methanol mango leaf extract/kg body weight, which demonstrates its broad spectrum of safety [24]. The extract contained approximately 4.25% mangiferin. The animals were observed for 14 days and no signs of gross toxicities including toxic manifestations or mortality were observed. Animals were also executed for gross anatomy check and macroscopic evaluation and no obvious organ changes were observed [24].

In a sub-chronic 90-day toxicity study, forty-eight male and female Sprague-Dawley rats (body weight 117-160 g) were orally administered either 0, 100, 300 or 900 mg of an aqueous ethanol mango leaf extract/kg body weight daily, which was equivalent to 17.2, 51.7 and 155.2 times the clinical daily dose (5.8 mg/kg), respectively, over a period of 90 consecutive days [23]. The extract contained 62% mangiferin. No significant abnormalities were observed in body weight and feed intake. All animals were normal at all doses with respect to coagulation functions, gross anatomy, hematology, biochemical criteria, and relative organ weights. Small but higher triglyceride and cholesterol levels were observed at the high dose (900 mg/kg) in male rats, and a lower potassium level was detected in female rats in the high dose group. Overall, no significant adverse events were observed [23].

Although no direct mutagenicity studies have been reported on mango leaf extract, based on its huge consumption by animals and humans around the world for several centuries, it can be considered non-mutagenic. A detailed investigation by Gold-Smith demonstrated the novel anti-cancer properties of mangiferin, which further supports its non-mutagenic potential [14].

Antioxidant Benefits of Mangiferin

Various research studies have demonstrated that mango leaf extract and mangiferin exhibit potent antioxidant and free radical scavenging activities, and the antioxidant potential is remarkably amplified in pro-inflammatory and inflammatory conditions, including infection and diabetic states [25-37]. The four hydroxyl groups in mangiferin demonstrate remarkable antioxidant properties by scavenging the noxious oxygen free radicals. As a potent iron chelator, it prevents the production of noxious hydroxyl radicals in Fenton-type reactions, as well as protects against UV (ultraviolet) radiation [18]. Using the DPPH assay, Dar et al. demonstrated the potent antioxidant activity of mangiferin (EC50 5.8±0.96 μg/ml) which is comparable to rutin, a potent antioxidant used for medical purposes [27]. It lowers hydrogen peroxide-induced lipid peroxidation in human peripheral blood lymphocytes in a dose-dependent manner. It prevents the depletion of GTP and total nucleotides as well as ATP damage and restores energy charge potential in H2O2-treated erythrocytes [25-29]. It also reduces diabetes-induced lipid peroxidation (TBARS) to a healthy level, as well as exhibiting antidiabetic activity [13,30].

Pardo-Andreu et al. showed that mangiferin works by a unique mechanism which is quite different from the classical hydroxyl radical scavengers, retaining iron in its original ferric form and by complexing Fe3+ or stimulating Fe2+ autoxidation [28-31]. However, Pokorski et al. in a hypoxic ventilator response model in rats indicated that the antioxidant properties superceded the iron chelating properties in this system [14]. Overall, the antioxidant potential of mangiferin counterbalances the iron-induced oxygen free radical formation, Fe2+ accumulation and iron-induced toxicity [28-31].

Various studies have affirmed the hepatoprotective effects of mango leaf extract and mangiferin. Karuppanan et al. demonstrated the hepatoprotective and antioxidant effects of mango leaf extracts against mercury chloride-induced liver toxicity in mice [32]. Extracts were shown to increase hepatic catalase, glutathione S-transferase and glutathione peroxidase levels. A methanolic extract was more effective than an ethanolic extract. Mangiferin has been shown to be a potent hepatoprotective against carbon tetrachloride-induced liver injury, which further supports its free radical scavenging activities [27]. Mangiferin protects against ethanol-induced hepatic damage [33]. Mangiferin also exhibited anti-apoptotic activity and anti-oxidant cytoprotection against glutaminergic excitotoxicity leading to neuronal death [34]. Arsenic-induced oxidative stress in the hepatic tissues and liver dysfunction was also dramatically protected by mangiferin in another study [32].

Xia et al. investigated the protective ability of mangiferin in a molecular model of H2O2-induced oxidative injury in osteoblast-like cultured MC3T3-E1 cells [35]. Mangiferin significantly reduced H2O2-induced apoptosis and oxidative stress in the MC3T3-E1 cells. The study demonstrated that mangiferin can protect these cultured MC3T3-E1 cells against oxidative injury by modulation of ERK5/Nrf2 signaling. These results suggest that mangiferin may be a potential agent in the intervention and prevention of osteoporosis [35].

Anti-Inflammatory Benefits of Mangiferin

A number of research studies have demonstrated that mangiferin exerts anti-inflammatory potential in various models. Pan et al. investigated the molecular mechanism of mangiferin against lipopolysaccharide (LPS) and D-galactosamine-induced acute liver injury and inflammation [36]. Mangiferin inhibited the levels of serum ALT, AST, IL-1β, TNF-α, MCP-1, and RANTES (regulated on activation, normal T cell expressed and secreted), as well as hepatic lipid peroxidation and oxidative stress. Mangiferin also inhibited IL-1β and TNF-α production in LPS-stimulated primary hepatocytes. Mangiferin up-regulated the expression of Nrf2 and HO-1 in a dose-dependent manner. Furthermore, mangiferin inhibited LPS/d-GalN-induced hepatic NLRP3, ASC, caspase-1, IL-1β and TNF-α expression. Thus, mangiferin protected against the liver injury by activating the Nrf2 pathway and regulating NLRP3 inflammasome activation [36].

Pardo-Andreu et al. have shown that mangiferin dose-dependently inhibited the inflammatory cytokines, TNFα, NO and NF-Кβ in both in vitro and in vivo models, affirming the anti-inflammatory potential of mangiferin [28]. Saha et al. postulated that the C-glucosyl linkage and hydroxyl groups in mangiferin contribute significantly to its free radical-scavenging activity. Furthermore, it modulates various transcription factors including NF-κΚB and Nrf-2, and expression of multiple pro-inflammatory signaling molecules including TNF-α and COX-2, which contribute to its anti-inflammatory benefits [17,37].

Agustini et al. demonstrated the protective abilities of mangiferin against doxorubicin (DOX)-induced cardiotoxicity and inflammatory responses in rats [38]. Mangiferin exhibited protection against DOX-induced cardiac inflammation and apoptosis via down-regulation of pro-apoptotic and pro-inflammatory gene expressions, upregulation of SERCA2a gene expression, and normalization of cytosolic calcium levels [38].

Wang et al. showed that mangiferin protected against renal ischemia-reperfusion injury by inhibiting inflammation, oxidative stress and inducing adenosine production in a mice model [39]. Mangiferin improved kidney function, increased CD73 expression in kidneys suffering from ischemia-reperfusion injury, enhanced adenosine production, and inhibited pro-inflammatory responses and tubular apoptosis [39].

Gong et al. demonstrated that mangiferin can attenuate cecal ligation and puncture-induced mortality and acute lung injury (ALI), as indicated by reduced systemic and pulmonary inflammatory responses as exhibited by reduced systemic and pulmonary inflammatory responses [40]. Mangiferin inhibited sepsis-activated mitogen-activated protein kinases (MAPK) and NF-Кβ signaling, demonstrating the inhibition of pro-inflammatory mediators. Furthermore, mangiferin dose-dependently up-regulated HO-1 in the pulmonary tissues of septic mice. It was further concluded that mangiferin attenuated sepsis by up-regulation of HO-1, which protects against sepsis-induced acute lung injury by modulating inflammatory responses [40].

Szandruk et al. assessed the protective ability of mangiferin in colon tissues against 2,4,6-trinitrobenzensulfonic acid (TNBS)-induced colitis in rodents [41]. Mangiferin reduced macroscopic and microscopic damage, as well as reduced MDA levels in the colon at 30 and 100 mg/kg body weight doses. Mangiferin (100 mg/kg body weight) also reduced TNF-α and IL-17 levels, and SOD activity in the colon tissues. The researchers concluded that mangiferin significantly protects against TNBS-induced inflammatory manifestations and colitis in rats by potentiating anti-inflammatory and antioxidant activities [41].

Gastrointestinal Transit and Gut Health

Human bacterial enzymes have been shown to metabolize mangiferin into phenolic acid metabolites in the colon, where these metabolites are capable of exhibiting biochemical and pharmacological effects [42]. Huang et al. demonstrated by using mass spectrometry and NMR that mangiferin can be metabolized in vitro by human intestinal bacteria into its corresponding aglycone 1,3,6,7-tetrahydroxyxanthene [42]. Bock and Ternes administered 74 mg mangiferin/kg body weight to pigs/day over a period of 11 days and feces were collected on day 11 and analyzed [43]. The researchers demonstrated that phenolic acids excreted in the feces included 3,4-dihydroxybenzoic acid, 3,4-dihydroxy-phenylacetic acid, 2,4,6-trihydroxybenzoic acid, and 3,4,5-trihydroxybenzoic acid following metabolism by intestinal microflora of animals treated with mangiferin.

In another study, Sanugul et al. identified the anaerobic bacteroides MANG which is involved in mangiferin metabolism to its corresponding aglycone through cleavage of its C-glucosyl bond [44]. MANG cleaved C-glucosyl in a dose- and time-dependent manner only when cultivated in the presence of mangiferin. However, mangiferin did not affect bacterial alpha- and beta-glucosidase activities under any conditions. The researchers pointed out that the C-glucosyl-cleaving enzyme substantially differs from known glucosidases that cleave O-glucosides. Furthermore, the researchers claimed that this is the first description of a specific intestinal bacterium that is involved in the metabolism of mangiferin and which produces a novel and inducible C-glucosyl-cleaving enzyme [44].

Helicobacter pylori-induced gastrointestinal injury including gastric cancer is a challenging problem and no appropriate therapeutic intervention has been developed. Zhang and Yue demonstrated that mangiferin has the ability to eradicate H. pylori and concurrently inhibit multiple inflammatory markers including NF-Кβ subunit p65, IL-1β, IL-8 and TNF-α . In addition, the protein expression of inflammatory enzymes as COX-2 and iNOS were remarkably down-regulated in cultured human gastric carcinoma AGS cells following incubation with mangiferin [45]. The researchers concluded that mangiferin significantly down-regulated the adhesion and invasion process as well as deactivated NF-p65, thereby blocking the inflammatory response and thus reducing the incidence of gastric carcinoma [45]. Interestingly, in several other studies, mangiferin has demonstrated inhibitory activity against Helicobacter pylori-induced inflammatory responses in the gastrointestinal tissues and gastrointestinal toxicity [42-44,46].

Morais et al. investigated the dose-dependent beneficial effects of mangiferin on gastrointestinal transit in normal and constipated mice and the underlying mechanism [47]. Overnight starved Swiss mice were orally administered 3, 10, 40 or 100 mg mangiferin/kg body weight or the drug tegaserod (1 mg/kg body weight, intraperitoneally) 30 min before a charcoal meal to assess the effect of mangiferin on normal transit. In the second set of experiments, the effect of mangiferin (30 mg/kg body weight) on delayed gastrointestinal transit induced by selected agonist(s) or antagonist(s) were assessed [47]. In the third set of experiments, mice were orally administered 3, 10, 40 or 100 mg mangiferin/kg body and 6 hour fecal pellet output was assessed. In addition, the ratio of wet to dry weight was calculated and used as a marker of fecal water content. The researchers concluded that mangiferin accelerated gastrointestinal transit in both normal and constipated mice and enhanced fecal output, but not water content in the fecal mass, while in the tegaserod group the fecal pellets had higher water content. The authors indicated that the increase in water content is indicative of augmented secretary activity. Overall, mangiferin was shown to have a safe prokinetic profile, since no diarrhea inducing effect was observed. The researchers concluded that mangiferin may serve as a functional agent to enhance regularity and ameliorate gastrointestinal disturbances [47]. Incidentally, an aqueous decoction of mango leaves enriched in polyphenols including mangiferin demonstrated potent antiulcerogenic potential [26].

Carvalho et al. investigated the gastroprotective efficacy of mangiferin against ethanol and indomethacin-induced gastric mucosal injury in mice. Mean gastric lesion areas and ulcer scores were determined [48]. Mangiferin significantly protected against ethanol-induced depletion of gastric mucosal non-protein sulfhydryl content in mice. Administration of mangiferin at 3, 10 and 30 mg/kg in mice decreased ethanol-induced gastrointestinal injury significantly by 30, 35 and 63%, respectively, and also reduced indomethacin-induced gastric injury [48]. Furthermore, mangiferin prevented gastrointestinal protein depletion in the gastric mucosa of rats.

Kakino et al. demonstrated a laxative effect and mechanism of an ethanolic extract of agarwood (Aquilaria sinensis, Aquilaria crasna) leaves, in which one of the major constituent is mangiferin. The authors demonstrated that these agarwood leaves enriched in mangiferin exerted a laxative effect via acetylcholine receptors in a mouse constipation model [49].

Wang et al. indicated that the therapeutic efficiency of mangiferin is restricted by its low intestinal permeability, and in order to improve the oral absorption of mangiferin, the authors assessed the comparative in vivo efficacy using a number of potential absorption enhancers including D-α-tocopherol polyethylene glycol 1000 succinate (TPGS), sodium deoxycholate and Carbopol 974P [50]. Increased oral absorption by the three co-excipients was in the order of Carbopol 974P > sodium deoxycholate > TPGS. The researchers concluded that these compounds are effective in enhancing the absorption of mangiferin in the gastrointestinal tract [50].

Mahmoud-Awny et al. investigated the molecular mechanism of antioxidant gastroprotective ability of mangiferin in a rat model of gastric ulcer. Modulatory effects on the signaling pathway were investigated using ischemia/reperfusion model in animals [51]. Mangiferin produced its gastroprotective benefits partly via inducing the expression of Nrf2, HO-1 and PPAR-γ along with down-regulating NF-Кβ . The antioxidant benefits were manifested by increasing total antioxidant capacity and reduced glutathione levels, besides normalizing (decreasing) MDA levels. Mangiferin reduced ischemia-reperfusion-induced NO dose dependently, enhanced endothelial nitric oxide synthase and also reduced the inducible isoform of nitric oxide synthase. Mangiferin exerted anti-inflammatory benefits by reducing serum IL-1β and E-selectin levels, effects that were mirrored in the tissue levels of myeloperoxidase, the neutrophil infiltration marker. Furthermore, mangiferin also exerted an anti-apoptotic effect by increasing Bcl-2 levels and reducing caspase-3 in a dose-dependent manner [51].

Another study investigated the gastroprotective benefits of a Mangifera indica leaf decoction in rodents. An aqueous decoction of Mangifera indica extract enriched in mangiferin exhibited significant gastroprotective benefits, including an antiulcerogenic activity [52]. Somani et al. investigated the molecular mechanism of mangiferin in a mouse model of colitis and inflammatory bowel disease (IBD) [53]. A molecular docking model and glide score demonstrated that mangiferin had significant binding potential with TNF-α and MMP-9. The researchers concluded that mangiferin reduced colonic injury in colitis by modulating oxidative and inflammatory parameters partly through attenuating the activity of TNF-α and MMP-9. Therefore, mangiferin may have potential in the management of IBD [53].

Morais et al. investigated the anti-inflammatory and prokinetic actions of mangiferin in a mouse model of post-operative ileus, as well as determined the potential ability of mangiferin to attenuate intestinal inflammation and impaired gastrointestinal transit [54]. These investigators concluded that mangiferin significantly inhibited the myeloperoxidase activity, a biomarker of neutrophil infiltration, and nitrate/nitrite levels in the ileum, and decreased the plasma levels of TNF-α, IL-1β, IL-6, and MCP-1. Thus, mangiferin significantly ameliorated intestinal inflammation and impaired gastrointestinal motility [54].

Dou et al. demonstrated that mangiferin significantly attenuated dextran sulfate sodium (DSS)-induced body weight loss, diarrhea, colon shortening and histological injury, which correlated with the decline in the activity of MPO and the levels of TNF-α in the colon [55]. DSS-induced degradation of IκBα and the phosphorylation of NF-Кβ p65 as well as the mRNA expression of pro-inflammatory mediator’s iNOS, ICAM-1, TNF-α, IL-1β and IL-6 in the colon were also down-regulated by mangiferin. Additionally, the phosphorylation/activation of DSS-induced MAPK proteins was inhibited. The researchers recommended that mangiferin may be utilized in the treatment of human IBD [55].

Herrera-Cazares et al. conducted an interesting investigation to develop a functional confectionery with two prime objectives: (1) retain the integrity of the phenolic antioxidants, and (2) enhance gastrointestinal absorption [56]. A confectionery product was formulated containing 20% mango bagasse, gelatin and pectin with a mangiferin content of 830.69 μg/g, a total polyphenol content 4.14 mg/g, a quercetin content of 244.83 μg/g, and a gallic acid content of 285.43 μg/g which was accessible in the intestine after 60-120 min of digestion. The results demonstrated that mangiferin exhibited increased bioaccessibility, absorption and antioxidant potential from this confectionery formulation [56].


A large body of historical Ayurvedic evidence indicates that mango leaf exerts diverse health benefits. A growing body of evidence involving animal, human clinical as well as in vitro studies supports the contention that mango leaf and mango leaf extracts exert diverse health-promoting benefits that may be as effective or superior to other modalities. This review summarizes the diverse health benefits of mango leaf extract enriched in mangiferin in promoting gastrointestinal protection, tissue protection, and glucose as well as lipid regulatory effects. It also eradicates noxious bacteria as Helicobacter pylori, and improves chronic renal and gut health [42,43]. Mangiferin exerts its effects by protecting the human body against free radical damage and oxidative stress, hence diminishing membrane damage and inflammation [57]. Additional research and clinical trials are warranted to confirm the diverse health benefits of mangiferin.


The authors thank Ms. Nipa Shah for technical assistance.

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Journal of Nutrition and Health Sciences

Figures at a glance
Figure 1
Figure 1: Structure of Mangiferin