Composition

EY (D1)

4%LDL(D2)

6%LDL (D3)

8%LDL (D4)

10%LDL (D5)

2.5%

11gm

16.5gm

22gm

27.5gm
Table 1: The concentrations of EY and LDL in the five extenders

Parameter

Group

Hour

Hr.0

Hr.3

Hr.24

Hr.48

Hr.72

Hr.96

Hr.120

Motility %

D1

 

D2

 

D3

 

D4

 

D5

 

 

83.50Aa ± 1.50

79.90Ba ± 9.9

53.0Bb ± 0.82
81.30Ba ±1.42

82.50Aa ±1.50

62.50Bc ±1.12

76.10Ca ± 1.07

36.0Cb ± 1.25
78.50Cac ± 1.50

 

80.30Bc ± 1.79

47.0Cd ± 1.11

59.50Da ±1.89

16.50Db ±1.30
64.0Dc ± 1.25

68.40Cd ±1.23

27.0De ± 1.11

43.0Ea ± 1.86
-
47.0Ea ± 2.0

 

52.50Db ±1.12

-

19.0Fa ± 1.45
-
28.0Fb ± 1.53

 

32.50Ec 1.12

-

-

 

-

-

17.5F ±1.12

-

Viability %

D1

 

D2

 

D3

 

D4

 

D5

 

 

84.20aA ± 1.62

80.35B
a±1.85

57.25B
b±1.01

81.62B
a±1.69

82.72B
a±1.72

65.63B
c±0.78

76.68Ca ± 1.45

40.53Cb ± 1.37

79.71Cac ±1.58

81.08Cc ± 1.59

52.30Cd ± 1.60

67.99Da ± 1.81

20.41Db ± 1.24

69.71Dac ± 1.09

 

73.39Dc ± 0.97

35.75Dd ± 1.23

48.61Ea ±2.01
-

 

51.68Ea ±1.79

60.0Eb ± 1.35

-

24.13F
a±1.29
-

 

31.16F
b±1.30

37.16F
c±1.17

-

-

-

-

 

27.18
G±1.2

-

Acrosomal integrity %

D1

 

D2

 

D3

 

D4

 

D5

 

81.96B
a±1.48

57.20B
b±0.97

81.99B
a±1.43

83.40B
a±1.49

53.10B
c±1.17

80.85Ba ± 1.41

35.45Cb ± 1.82

80.61Ca ± 1.40

81.53Ca ± 1.46

41.10Cc ± 1.16

75.73Ca ± 1.56

16.97Db ± 1.13

69.04Dc ± 1.03

73.91Da ± 0.74

23.15Dd ± 0.78

55.34Da ±1.67
-
51.14Ea ±1.58

60.55E
b±1.09
-

37.79Ea ± 1.04
-
29.64Fb ± 1.41
44.50Fc ± 1.27
-

-

 

-

-

22.05
G±1.5
-

Plasma membrane integrity %

D1

 

D2

 

D3

 

D4

 

D5

 

 

83.38Aa ± 1.42

79.80B
a±1.44

59.76B
b±0.72

81.44A
a±1.10

81.37A
a±1.43

63.37Bc ± 1.0

74.25Ca ± 1.36

37.07Cb ± 1.23

77.08Bc ± 1.58

78.75Bc± 1.28

49.93Cd ± 1.54

63.78Da ± 1.63

17.57Db ± 1.33

64.78Ca ± 1.16

70.64Cc ± 1.36

31.93Dd ± 1.15

44.05Ea ±2.02
-

 

46.87Da ±1.60

54.25D
b±1.19

-

21.0Fa ± 1.40
-

 

26.43E
b±1.22

33.85E
c±1.10

-

Sperm abnormalities

D1

 

D2

 

D3

 

D4

 

D5

 

 

9.66Aa ± 1.0

15.49B
a±1.18

31.97B
b±1.17

14.61B
a±0.66

11.77Ac ± 0.63
17.72B
d±0.71

17.12Ba ± 1.05

36.41Cb ± 1.20

19.22Ca ± 0.71

15.74Bc ± 0.73

 

24.70Cd ± 1.16

19.67Ca ± 0.87

40.78Db ± 1.21

22.09Da ± 0.58

19.73Ca ± 0.56

38.95Db ± 0.91

33.85Da ±0.98

-

 

31.41Ea ±0.94

27.21D
b±0.84

-

43.28E
a±0.85

-

 

41.58F
a±0.95

36.35E
b±0.81

 

-
Mean ± SEM in same row with different capital letters are significantly different (p<0.05)
Mean ± SEM in same column with different small letters are significantly different (p<0.05)
Dots (-) indicate sperm became non-motile
Table 2: Effect of different LDL concentrations on parameters of cooled buck semen