Adenovirus (Source: CDC)

Figure 1: a) Uninfected epithelial cell b) Early infected epithelial cell c) Late infected epithelial cell

(Source: Courtesy of Linda Stannard, University of Cape Town, S.A.)

Figure 2: a) Cytopathic effect of HSV on cell culture b) Cytopathic effect of CMV c) Adenoviral inclusion bodies

Figure 3: Positive immunofluorescence tests for a) HSV antigen from epithelial cell b) CMV pp65 antigen from peripheral blood neutrophils and c) Test for rabies virus antigen (Source: CDC)

	Type of virus	Cell lines
1	Herpes Simplex	Vero, Hep-2, human diploid (HEK-293 and HELA), human amnion
2	VSV	human diploid (HELA, HEK)
3	CMV	human diploid fibroblasts (HEF)
4	Adenovirus	Hep-2, HEK,
5	Poliovirus	MK, BGM, LLC-MK2, Vero, Hep-2, Rhadomyosarcoma, HELA, HEK
6	Coxsackie B	MK (Monkey Kidney cell), BGM, LLC-MK2, Vero, hep-2
7	Echo	BGM (Buffalo green monkey), MK, LLC-MK2, human diploid, Rd
8	Influenza A	MK, LLC-MK2, MDCK(Madin-Darby canine kidney cells)
9	Influenza B	MK, LLC-MK2 (Rhesus Monkey Kidney Epithelial Cells), MDCK
10	Parainfluenza	MK, LLC-MK2
11	Mumps	MK, LLC-MK2, HEK, Vero
12	RSV	Hep2 (Human epidermoid cancer cells), Vero
13	Rhinovirus	human diploid (HEK, HEL (Henrietta Lacks))
14	Measles	MK, HEK(Human embryo kidney), Vero, B95a
15	Rubella	Vero, RK13 (Rabbit kidney epithelial cells)
16	Rabies	WI-38 (Human lung fibroblast cell line), human diploid cells
17	HCV	(Huh-7) human hepatocellular carcinoma cell line
Table 1: Some selected viruses and their cell cultures

Name of Virus	Cell Culture	Origin of cell	Specimen type	Incubation period (CPE)	Storage temperature
CMV	HEL	Human embryonic lung fibroblasts	CSF/Blood	5 - 14 days	-70 °C to -196 °C
Poliovirus(1,2,3)	Rhadomyosarcoma (Rd)	Human cancer of connective tissue	Stool	1 - 7days	-86 °C to -196 °C
VZV	HeLa	Human fibroblasts	Vesicle fluid and scrapings	5 - 14 days	-70 °C to -196 °C
RSV	HeP-2	Human cervical cancer cells	Nasopharyngeal aspirates, nasal washes	4 - 5 days	-70 °C to -196 °C
Influenza A	MDCK	Madin-Darby canine kidney cells	Throat swab	2 - 5 days	-70 °C to -196 °C
HSV	HEK-293	Human diploid cell	Genital and tissue lesions	1 - 4 days	-70 °C to -196 °C
Adenovirus	HEK	Human embryo kidney	Stool/eye swab	2 - 7 days	-80 °C to -196 °C
Mumps	MK	Monkey kidney cell	pharyngeal exudates, saliva	3 - 10 days	-20 °C to -70 °C
Rubella virus	RK-13	Rabbit kidney cell	Dermal lesion	3 -10 days	-70 °C to -196 °C
Measles	MK	Monkey kidney cells	Blood, throat swabs	3 - 10 days	-70 °C to -196 °C
Rabies	WI-38	Human lung fibroblast	Neck skin biopsy	5-14 days	-20 °C to -80 °C
Rhinovirus	HeLa	Human fibroblast	Nasal swab	4-5 days	-20 °C to -196 °C
(Source: https://www.mayomedicallaboratories.com/.html) Table 2: Different viruses with their culture, specimen type incubation period and storage temperature